double staining apoptosis kit (Vazyme Biotech Co)
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double staining apoptosis kit
Double Staining Apoptosis Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 2339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+staining+kit/Annexin+V-FITC+PI+Apoptosis+Detection+Kit/pmc13022693-98-5-9
Average 97 stars, based on 2339 article reviews
Double Staining Apoptosis Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 2339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+staining+kit/Annexin+V-FITC+PI+Apoptosis+Detection+Kit/pmc13022693-98-5-9
Average 97 stars, based on 2339 article reviews
double staining apoptosis kit - by Bioz Stars,
2026-09
97/100 stars
Images
1) Product Images from "WTAP stabilizes MMP12 expression to promote the malignant phenotypes of esophageal cancer cells"
Article Title: WTAP stabilizes MMP12 expression to promote the malignant phenotypes of esophageal cancer cells
Journal: Regenerative Therapy
doi: 10.1016/j.reth.2026.101101
Figure Legend Snippet: Screening of genes in EC. (A) The Venn diagram shows the intersection between the DEGs in EC and normal esophageal tissues and the genes related to apoptosis, proliferation, and glycolysis. (B-D) The Lasso regression, SVM, and RF algorithms were used to further screen the 11 genes and identify key signature genes. (E) The Venn diagram shows the key genes identified by the Lasso regression, SVM, and RF algorithms.
Techniques Used:
Figure Legend Snippet: MMP12 knockdown inhibited the migration, invasion, proliferation, and glucose metabolism and induced cell apoptosis. KYSE150 cells were transfected with si-MMP12 or si-NC. (A and B) Cell migration and invasion were analyzed by transwell assays. (C) Cell proliferation was analyzed by EdU assay. (D) Cell apoptosis was assessed by flow cytometry. (E) HK1 and LDHA protein expression were detected by Western blotting. (F–H) Glucose consumption, lactate production, and ATP levels were analyzed by commercial kits. ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
Techniques Used: Knockdown, Migration, Transfection, EdU Assay, Flow Cytometry, Expressing, Western Blot
Figure Legend Snippet: WTAP knockdown inhibited the migration, invasion, proliferation, and glucose metabolism and induced cell apoptosis by regulating MMP12 expression. KYSE150 cells were transfected with si-WTAP, MMP12 overexpression plasmid, or the matched control (si-NC and oe-NC). (A) MMP12 protein expression was detected by Western blotting. (B and C) Cell migration and invasion were analyzed by transwell assays. (D and E) Cell proliferation was analyzed by EdU assay. (F) Cell apoptosis was assessed by flow cytometry. (G and H) HK1 and LDHA protein expression were detected by Western blotting. (I–K) Glucose consumption, lactate production, and ATP levels were analyzed by commercial kits. ns: not significant, ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
Techniques Used: Knockdown, Migration, Expressing, Transfection, Over Expression, Plasmid Preparation, Control, Western Blot, EdU Assay, Flow Cytometry
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Article Title: WTAP stabilizes MMP12 expression to promote the malignant phenotypes of esophageal cancer cells Article Snippet: Flow cytometric analysis was performed with an LSRII instrument (BD Biosciences, San Jose, CA, USA). .. Apoptosis was evaluated using a Article Title: The ASFV CD2v protein inhibits apoptosis by inducing proteasomal degradation of Bim EL via activation of the TPL2-MEK-ERK signaling pathway Article Snippet: .. The cell suspensions were combined and analyzed for apoptosis by flow cytometry following double-staining with Article Title: The ASFV CD2v protein inhibits apoptosis by inducing proteasomal degradation of Bim EL via activation of the TPL2-MEK-ERK signaling pathway. Article Snippet: .. The cell suspensions were combined and analyzed for apoptosis by flow cytometry following double-staining with TUNEL Assay:Article Title: Hydroxyl radical from iridium(III)-based photosensitizer triggers PANoptosis and ferroptosis for hypoxia-tolerant photodynamic immunotherapy. Article Snippet: Exploring photosensitizers that efficiently generate oxygen-independent hydroxyl radical (•OH) and activate highly immunogenic PANoptosis and ferroptosis shows promise in addressing the limited efficacy of photodynamic immunotherapy (PDI) in the hypoxic and immunosuppressive tumor microenvironment (TME).. However, challenges still exist due to the unsatisfactory •OH production efficiency in most organic photosensitizers and the unresolved relationship between •OH and PANoptosis.. Here, we designed and synthesized two novel iridium(III) complexes, Ir1 and Ir2. Glutathione Assay:Article Title: Hydroxyl radical from iridium(III)-based photosensitizer triggers PANoptosis and ferroptosis for hypoxia-tolerant photodynamic immunotherapy. Article Snippet: Exploring photosensitizers that efficiently generate oxygen-independent hydroxyl radical (•OH) and activate highly immunogenic PANoptosis and ferroptosis shows promise in addressing the limited efficacy of photodynamic immunotherapy (PDI) in the hypoxic and immunosuppressive tumor microenvironment (TME).. However, challenges still exist due to the unsatisfactory •OH production efficiency in most organic photosensitizers and the unresolved relationship between •OH and PANoptosis.. Here, we designed and synthesized two novel iridium(III) complexes, Ir1 and Ir2. ATP Assay:Article Title: Hydroxyl radical from iridium(III)-based photosensitizer triggers PANoptosis and ferroptosis for hypoxia-tolerant photodynamic immunotherapy. 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Apoptosis was evaluated using a Flow Cytometry:Article Title: The ASFV CD2v protein inhibits apoptosis by inducing proteasomal degradation of Bim EL via activation of the TPL2-MEK-ERK signaling pathway Article Snippet: .. The cell suspensions were combined and analyzed for apoptosis by flow cytometry following double-staining with Article Title: The ASFV CD2v protein inhibits apoptosis by inducing proteasomal degradation of Bim EL via activation of the TPL2-MEK-ERK signaling pathway. Article Snippet: .. The cell suspensions were combined and analyzed for apoptosis by flow cytometry following double-staining with |
